Food Contamination: Quality, Safety & Authenticity, Thermo Scientific Varioskan LUX Multimode Microplate Reader, Atomic Absorption / Emission Spectroscopy, Integrated Assay Prep / Analysis Workstations. Whether you are new to western blotting or an experienced researcher wanting to confirm your knowledge, consider this center to help you to get better western results and succeed sooner. 0000144246 00000 n The Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mix of eight purified recombinant proteins ranging from 60K to 250K. The Mark12 Unstained Standard allows the closest estimation of molecular weight of your sample protein over a wide molecular weight range. 0000004109 00000 n For Research Use Only. 78k w7 We stand behind the performance of our protein gels. For Research Use Only. 0000014967 00000 n 0000002879 00000 n Generate quality protein samples for separation with optimized tissue lysis, cell lysis, and sub-cell fractionation buffers. Achieve your best protein electrophoresis and western blotting results. Thermo Fisher Scientific Inc. is the world leader in serving science. Don't have an account ? Find the exact gel and buffers needed for your electrophoresis experiments. Optimized for analysis of a wide range of protein targets, Prestained, unstained and western blot ladders, Stains for every application from quick visualization to high-sensitivity applications, Quality transfers using less volume of methanol-based transfer buffers, Mini, midi gel electrophoresis, and transfers. Search 0000005895 00000 n 0000096054 00000 n Use No-Stain Protein Labeling Reagent for total protein normalization to help improve the accuracy of your quantitative western blotting data. The Mini Gel Tank design enables convenient side-by-side gel loading and enhanced viewing during use. Find our E-Gel selection table that will guide you in choosing the best products for your experiments. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing visualization of the standard on the western blot. 0000094292 00000 n Avaialble in a variety of sizes:  Read more Bond-Breaker TCEP Solution is a stable thiol-free, phosphine-based TCEP compound useful as a replacement for Beta-mercaptoethanol or DDT in SDS-PAGE sample buffers. Search Thermo Fisher Scientific. 0000144122 00000 n Invitrogen E-Gel Power Snap Plus Electrophoresis System combines the convenience of quick real-time nucleic acid analysis with high-quality visualization in both high- and low-throughput Invitrogen E-Gel products. Explore our protein gel options. 0000001706 00000 n This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications, and how to introduce the right new gel chemistry in your research workflow. The choice of whether to use one chemistry or another depends on the abundance of the protein youre separating, the size of the protein, and your downstream application. 0000009304 00000 n The iBlot 2 Transfer Stacks are consumable stacks with integrated PVDF or nitrocellulose transfer membranes for dry blotting of proteins using the iBlot 2 Transfer Device. 0000002442 00000 n Our extensive portfolio of precast protein gels provides a wide variety of gel options available for your research. Find the right gel for your research needs based on molecular weight, downstream applications, and throughput requirements. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI. Direct visualization can be achieved through standard staining protocols (e.g. Load up to 60 L per well with Bolt gels. Thermo Fisher Scientific, Discover the new ways to do western blotting with our how-to videos, Overcome western blot difficulties with our troubleshooting guidance, Gain quick tips to better western blots and data, Watch webinars to receive firsthand knowledge of the latest technologies for western blotting, Find recipes for buffers, stock solutions, and general western blot protocols, Tech tips, application notes, handbooks and more. The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer). Invitrogen iBlot 2 Transfer Stacks, nitrocellulose, Invitrogen SeeBlue Plus2 Pre-stained Protein Standard, Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, Thermo Scientific Pierce Silver Stain Kit, Vybrant DyeCycle Green and Orange Stains, Invitrogen NuPAGE Bis-Tris Protein Gels, Thermo Scientific Owl EasyCast B2 Mini Gel Electrophoresis Systems, Classic Series 96 Well Compatible Gel Systems, Thermo Scientific Bond-Breaker TCEP Solution, Neutral pH, Thermo Scientific Spectra Multicolor High Range Protein Ladder, Thermo Scientific Spectra Multicolor Broad Range Protein Ladder, Invitrogen NativeMark Unstained Protein Standard, Thermo Scientific PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa, Invitrogen MagicMark XP Western Protein Standard, Corporate Social Responsibility Statement. 0000007644 00000 n Not for use in diagnostic procedures. Protein gels performance guaranteeInvitrogen precast protein gels are designed and tested to deliver superior performance, reliability, reproducibility, and consistency of quality. Learn how the right protein gel chemistry can help you achieve better protein separation and western blots. There is even a jigsaw puzzle to solve on the back cover of each book. Protein gel electrophoresis is a simple way to separate proteins prior to downstream detection or analysis. Protect samples with convenient and effective protease and phosphatase inhibitors, ideal for the protection of proteins during extraction or lysate preparation. 0000012693 00000 n This helps reduce protein modifications and helps produce sharp band resolution. 0000010044 00000 n Read more "Twist gene fragments are great value and easy to use.". Neutral pH for better separation, transfer, and analysis of high molecular weight proteins. 0000097009 00000 n For separation of high molecular weight proteins, Tris-acetate gel chemistry is recommended, offered in NuPAGE Tris-Acetate gels. Powered by Madgex Job Board Software. Read more NuPAGE Bis-Tris gels are precast polyacrylamide high-performance gels that offer optimized chemistry for denaturing gel applications. Western blotting, in-gel staining, samples containing detergent and high salt, and denaturing or native PAGE applications. With the built-in timer, you can challenge your friends and labmates to solve it faster than you. Not for use in diagnostic procedures. U$2@1"k6)O ]+~^lZ?6X wF endstream endobj 128 0 obj <>stream Check out our Certificate of Analysis. The protein markers consists of recombinant proteins with an IgG binding site. 0000093852 00000 n Both models are perfect for DNA/RNA submarine electrophoresis. Western blotting, in-gel staining, mass spectrometry, post translationally modified proteins, dilute samples, and denaturing applications. Our precast protein polyacrylamide gels are offered in four different chemistries. ROCKFORD, Ill. (January 16, 2012) Thermo Fisher Scientific, the world leader in serving science, today announced the addition of two new protein molecular weight markers for determining the size of proteins in gel electrophoresis and Western blotting applications. View upcoming and recorded webinars for in-depth understanding of molecular biology techniques and resources. For denaturing gel electrophoresis or SDS PAGE there are many options available based on the application, and size of the protein of interest. Designed to give you total felxibility in your application. Thermo Fisher Scientific. For separation of low molecular weight proteins, use Novex Tricine gels which provide increased resolution of proteins with molecular weights as low as 2.5 kDa. You choose the degree of difficulty. The purpose of this center is to connect scientists to our many resources for learning about protein gel electrophoresis and western blotting methods and products. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. xb```c``0AA2,&[+c`[7i]`5-\uJl* Have questions about the E-Gel system or new to molecular biology applications? Accelerating generation of single cell clones by using CellRaft AIR System coupled with Cellular assay using brightfield and fluorescence-based live cell imaging. 0000008463 00000 n 0000004988 00000 n HI}K 0000096644 00000 n 0000006775 00000 n The 105 and the 250-90 are simple to use, lightweight, multipurpose low voltage power supplies. This video will show you how to separate proteins by using Invitrogen Mini Gel Tank and Invitrogen precast SDS-PAGE gels. Request sampleInteractive selection tool, PageRuler Prestained Protein LadderClick image to enlarge, PageRuler Plus Prestained Protein LadderClick image to enlarge. However, gaining publication quality results straightaway is not exactly the norm when performing western blotting. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers. Don't have an account ? a/$dLPg5vULx1qa+sN>FJJ!oT,RMRtKZzd78".`PF%%c0 M 4}} f$ lL'c11dz pdHD'pmc ` X'WE< L The Pierce Silver Stain Kit is a rapid, ultra-sensitive and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results with many 1D and 2D gel types Read more Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. l99Dz%kJ&Y/IG.$l1,K)Z9T?@`23@b%@N` vi$OX$-=y6 qQBtg.$PZR^;W 8#T9?3Th_We Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Thermo Fisher Scientific. The ready-to-use Thermo Scientific PageRuler NIR Protein Ladder contains a mixture of 10 proteins ranging from 11K to 250K. Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Microbiological Media and Media Additives, Gel Electrophoresis Equipment and Supplies, NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels, Recommended Well Loading Volumes & Sample Loads, Protein Electrophoresis Buffers and Reagents, Protein Gel Electrophoresis Chamber Systems, Protein gel electrophoresis technical handbook, Protocols, tips & tricks, and electrophoresis troubleshooting, NuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus Gels. Not for use in diagnostic procedures. All prestained protein ladders are compatible for use in western blotting. Get started with our money-saving Protein Gels Welcome Packs, Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell, Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only). 0000002993 00000 n When used to its full capacity, the Electro-Fast can accommodate samples from eithe Read more DNA-selective, cell membrane-permeant, and nonfluorescent stains for DNA content analysis in living cells. Don't have an account ? Watch fun and educational videos on molecular biology techniques, how-tos, and tips and tricks. HUjI}G)08lv!DE -~] FuSk&7gVY'.gjL`zzmR$\yY700e> $gC,E!$YGl/M BsL=kd[cF% L,S)#O '%!f)%zk&=7s6S46!f[r>|T5,=NIJIAGcW[~uLVP] Precast gels with a gradient can be used or gels can be hand cast. Read more Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green-and orange-stained proteins (40 to 300kDa) for use as size standards for high-MW proteins in gel electrophoresis and Western blotting. 0000094217 00000 n trailer <<5FC37DBA592A4C0ABE8F5A80CB866F52>]>> startxref 0 %%EOF 157 0 obj <>stream 0000096320 00000 n Neutral pH helps prevent protein degradation for high sensitivity. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separation of native proteins, neutral pH for high sensitivity applications, Separation of proteins based on isoelectric point, Separation of proteins based on size and activity, High-throughput gel system, that eliminates the need for buffers, SureCast reagents, other popular polyacrylamide reagents, Western blot transfer and analysis, and all techniques in which protein integrity is crucial, Mini with larger well capacity (WedgeWell, up to 60 L), Broad-range separation of proteins using traditional Laemmli-style gels, Separation of small proteins and peptides, High throughput for recombination product analysis and protein profiling, Leak-free designminimize the number of failed gels, waste less time, Unique tilt featureminimize spillage and confidently load acrylamide solutions, Simple component assemblyusing a single-motion load-and-lock mechanism, Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage), Special coating provides uniform polymerization rate for a more consistent handcast gel, High capacity sample wellsload up to 60 L per well. The ladder is ready to use without heating and diluting. Three simple steps: load, run and analyze. Not for use in diagnostic procedures. Room temperature storage for up to 16 months. Our portfolio of high-quality protein electrophoresis products unites gels, gel tanks, protein gel handcast system, stains, molecular weight markers and standards, running buffers, and blotting products for your protein analysis experiments. 0000001604 00000 n 0000096617 00000 n 0000143998 00000 n For easy reference, the 55K protein band has greater intensity than the other ladder bands. For separation of a broad range of proteins, two chemistries, Bis-Tris and Tris-glycine, have been optimized for performance and long shelf life. HlUK#9)V >B:xo&Urt 8mqJO. Create Account, SDS-PAGE buffers, native buffers and reagents for gel casting, sample preparation, running and transferring gels, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Microbiological Media and Media Additives, Gel Electrophoresis Equipment and Supplies, Protein Electrophoresis Buffers and Reagents, Protein Gel Electrophoresis Chamber Systems, Protein Biology Equipment and Plasticware, Protein Purification and Analytics Interactive Decision Tool, Total Protein Normalization for gel or blots, tissue lysis, cell lysis, and sub-cell fractionation buffers, Protein gel electrophoresis technical handbook, Protocols, tips & tricks, and electrophoresis troubleshooting. Bolt Bis-Tris Plus mini gels build on the legacy of the highly published NuPAGE Bis-Tris gels, feature larger capacity wells- for loading up to 60 l per well, and can be run in as little as 20 minutes. To separate high-abundance proteins, select our robust Novex Tris-Glycine gel chemistry, which offers maximum protein separation performance and crisp, straight bands. 0000109261 00000 n 0000003105 00000 n For Research Use Only. ,YX.0Qc7L^4D0 2`F H endstream endobj 120 0 obj <>/Metadata 117 0 R/Pages 116 0 R/Type/Catalog>> endobj 121 0 obj <>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC]/Properties<>/MC1<>>>/ExtGState<>>>/Type/Page>> endobj 122 0 obj <> endobj 123 0 obj <> endobj 124 0 obj [/ICCBased 135 0 R] endobj 125 0 obj <> endobj 126 0 obj <> endobj 127 0 obj <>stream For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. We guarantee it. Thermo Fisher Scientific. The 150K band of the ladder has greater intensity for easy orientation. No other supplier conducts such stringent quality testing. Search Electrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Download: Protein gel electrophoresis technical handbook, Download: Western Detection Workflow brochure, Access: Protocols, tips & tricks, and electrophoresis troubleshooting. Choose from precast polyacrylamide gel electrophoresis (PAGE) chemistries designed for specific applications including broad range, high, or low molecular weight separations, SDS-PAGE, native PAGE, or IEF. For more information about the new PageRuler NIR Protein Ladder and the PageRuler Unstained High Range Protein Ladder products, please visit www.thermoscientific.com/pierce. 0000109931 00000 n %PDF-1.6 % Don't have an account ? Fast 2035 minute run times. Read more PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. commassise stains), or indirect through chromogenic, chemiluminescent, or fluorescent detection systems. Superladders derived from cloned and purified DNA fragment repeats, for quantitation and accurate sizing 2. For Research Use Only. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. 0000138581 00000 n Whether your protein is 2.5 kDa or as large as 500 kDa, or youre looking for separation under native conditions or by isoelectric pointwe have the right gel for your application. Western blot analysis is one of the most common methods used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample and revealing their activity and any post-translational modifications. 119 0 obj <> endobj xref 119 39 0000000016 00000 n Read more Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting. Access literature, selection guides, webinars, and additional educational resources below for more information. There are no gels to pour, buffers to make, or staining neededfor ultimate convenience. For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. Don't have an account ? Find the right gel for your proteinConvert from other suppliers' gels. Traditional Laemmli-style gels with larger loading capacityload up to 60 L per well. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Download: Protein gel electrophoresis technical handbook, Download: Western Detection Workflow brochure, Access: Protocols, tips & tricks, and electrophoresis troubleshooting. Mini gels and wider, higher throughput, midi gels are offered in a variety of well and cassette formats. Choose Bis-Tris gel chemistry when you have a low abundance of protein or when your downstream applications require high protein integrity, such as posttranslational modification analysis, mass spectrometry, or sequencing. /o(0gTjfNPx#sCFB All Invitrogen precast protein gels are high quality polyacrylamide gels designed to deliver superior performance, reliability, reproducibility, and consistency of quality. Search It's that simple. Read more Thermo-Fast Electro-Fast Gel Systems are ideal for high throughput electrophoresis of PCR products or samples containing a small number of DNA species. Create Account. The Thermo Scientific 1kb DNA Ladder is and easy reference ladder with brighter bands at 1kb and 3kb.